Standardizing human peripheral blood mononuclear cell preservation and analysis
GVL Organizing Committee: Anita Chong (U Chicago, USA), Ed Geissler (U Regensburg, Germany), Megan Levings (UBC, Canada), Jonathan Maltzman (U Pennsylvania, USA), Birgit Sawitzki (Charite U, Germany), Qizhi Tang (UCSF, USA), Stefan Tullius (Harvard, USA)
9-11:30 AM, 11th November 2015, Lorne, Australia (preceding the Transplantation Science Symposium)
Learn from an international panel of experts on how to get the most out of your clinical blood samples for phenotypic and function analysis. Bring your questions and experiences and participate in a Q&A discussion session.
- Learn the most effective way of collecting, freezing and thawing human peripheral blood cells.
- Identify and overcome variables that affect the quality of human T cell ELISPOT assays.
- Understanding the complexities of multi-color flow cytometry with human blood samples.
|Chairs: Ed Geissler (University of Regensburg, Germany); Anita Chong (University of Chicago, USA)|
|09:00-09:05||Welcome and introductory remarks|
|09:05-09:35||Qizhi Tang, Ph.D., University of California San Francisco, USA
Optimizing the collection, freezing and recovery of peripheral blood mononuclear cells
|09:35-10:05||Robert Carroll, MD, The University of Adelaide, Australia
Standardizing human T cell Elispot assays
|10:05-10:35||Mathias Streitz, PhD, Charite University Medicine, Germany
Panel design and standardization of multi-parametric flow cytometry
|10:35-11:05||Megan Levings, PhD, University of British Columbia, Canada
Implementation of standardized multi-parametric flow cytometry in multi-centre clinical trials
|11:05-11:30||Q&A and Panel Discussion
Robert Carroll, Sabine Ivison, Megan Levings, Mathias Streitz, Qizhi Tang, Stefan Tullius
The workshop has a cost of $55.00 USD. To purchase it, you will have to register. Visit our registration page for more information.